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牙龈、牙周膜、牙髓组织体外培养结果只有纤维细胞生长,培养成功率分别为20/20、4/20和4/52,最高传代代数为50、20和18,最高传代者生长稳定期为5～40、5～15和5～12代,生长稳定期倍增时间为44、70和90h。在倒置和光学显微镜下观察3种来源的细胞无明显差别,电镜观察显示牙龈纤维细胞两端有许多微细突起,细胞器较发达,含丰富糖原颗粒;牙周膜纤维细胞两端微细突起较少,细胞中糖原颗粒较多,有空泡样结构;牙髓纤维细胞两靖微细突起少,细胞中糖原颗粒少,空泡样结构多。
Gingival, periodontal ligament, pulp tissue culture results in vitro only fibroblast growth, culture success rates were 20/20, 4/20 and 4/52, the highest pass generation algebra was 50,20 and 18, the highest pass on the growth of stable phase For 5 ~ 40, 5 ~ 15 and 5 ~ 12 generations, the doubling time of growth stability was 44, 70 and 90 h. There was no significant difference between the three kinds of cells observed under inverted and light microscope. Electron microscopy showed that there were many fine protrusions on both ends of gingival fibroblasts, the organelles were more developed and rich in glycogen granules. The number of fine protrusions on both ends of periodontal ligament fibroblasts was less , More glycogen granules in cells, vacuole-like structure; two Jing Jing pulp small protrusions, less glycogen particles in the cell, vacuole-like structure and more.