聚丙烯酰胺凝胶电泳法(polyacrylamide gel electrophoresis,PAGE)是目前对蛋白质、抗体、核酸等生物大分子进行分离、纯化及分子量测定的常用主要方法之一。十二烷基磺酸钠(SDS)是一种阴离子去污剂,它可以断开分子内和分子间的氢键,破坏蛋白质分子的二级和三级结构。在蛋白质中加入强还原剂β-巯基乙醇(β-mercaptoethanol,BME)可以断开半胱氨酸残基之间的二硫键。待测蛋白样品在结合大量的SDS后,各组分之间的形状和电荷差异被抵消,在电场中泳动的 Polyacrylamide gel electrophoresis (PAGE) is one of the most commonly used methods for the isolation, purification and molecular weight determination of biological macromolecules such as proteins, antibodies and nucleic acids. Sodium dodecyl sulfate (SDS) is an anionic detergent that breaks down intramolecular and intermolecular hydrogen bonds and disrupts the secondary and tertiary structures of protein molecules. Adding a strong reducing agent, β-mercaptoethanol (BME) to the protein breaks the disulfide bonds between cysteine ​​residues. After binding a large amount of SDS to the protein sample to be tested, the difference in shape and charge between the components is canceled and the electrophoretic