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目的:探讨氧化苦参碱(oxymatrine,OM)抑制人结肠癌LoVo细胞增殖和诱导凋亡的分子作用机制。方法:采用流式细胞仪检测LoVo细胞凋亡率以及细胞周期分布;采用荧光定量PCR法检测0.25,0.5 g.L-1 OM对LoVo细胞增殖相关基因c-myc,蛋白酶调解因子9(PSMD9),CDK4的基因表达的影响。结果:0.5 g.L-1以下浓度的OM作用结肠癌LoVo细胞48 h,对细胞凋亡无明显影响。0.25 g.L-1 OM作用48 h时可明显抑制人结肠癌LoVo细胞c-myc基因表达(P<0.05)。0.5g.L-1 OM作用48 h时可明显抑制LoVo细胞c-myc,CDK4的基因表达(P<0.01,P<0.01,)。药物作用时间为96 h时,0.5g.L-1 OM可明显抑制c-myc,PSMD9,CDK4基因表达(P<0.05,或P<0.01)。结论:较低剂量OM显著抑制人结肠癌LoVo细胞增殖的作用机制,可能与下调LoVo细胞c-myc,PSMD9,CDK4表达有关。
AIM: To investigate the molecular mechanism of oxymatrine (OM) inhibiting the proliferation and inducing apoptosis of human colon cancer LoVo cells. Methods: The apoptosis rate and cell cycle distribution of LoVo cells were detected by flow cytometry. The expression of c-myc, PSMD9, CDK4 The impact of gene expression. Results: LoVo colon cancer cells treated with OM at a concentration of 0.5 g.L-1 for 48 h had no significant effect on apoptosis. The effect of 0.25 g.L-1 OM for 48 h on c-myc gene expression in human colon cancer LoVo cells was significant (P <0.05). The effect of 0.5g.L-1 OM for 48 h on the gene expression of c-myc and CDK4 in LoVo cells was significant (P <0.01, P <0.01). 0.5g.L-1 OM significantly inhibited the gene expression of c-myc, PSMD9 and CDK4 (P <0.05 or P <0.01) at 96 h. Conclusion: The effect of OM at low dose on the proliferation of human colon cancer LoVo cells may be related to the down-regulation of c-myc, PSMD9 and CDK4 expression in LoVo cells.