酶组织化学的双重染色技术是指在同一切片上进行两种酶反应,从而在同一组织内显示两种酶的定位及活性。其意义在于更清晰地显示不同组织结构,在疾病情况下可同时显示不同细胞器或不同酶活性的损伤。本实验用大鼠及人的不同器官或组织的未固定及不同固定液固定后的恒冷切片作双重酶反应,为国内开展有关技术提供参考资料。 The double staining technique of enzymatic histochemistry refers to performing two enzyme reactions on the same slice to show the localization and activity of the two enzymes in the same tissue. The significance lies in the clearer display of different organizational structures, in the case of disease can simultaneously show different organelles or different enzyme activity damage. In this experiment, rat and human organs or tissues of different fixed and different fixed fluid immobilized frozen double-enzyme reaction for the domestic to carry out the relevant technology to provide reference materials.