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目的:探讨隐丹参酮对TNF-α诱导的人脐静脉内皮细胞(HUVEC)粘附分子ICAM-1表达的影响及机制。方法:通过建立TNF-α诱导的HUVEC细胞炎症模型,以抗氧化剂吡咯烷二硫代氨基甲酸盐(PDTC)做为阳性对照,用细胞酶联免疫吸附法、免疫荧光法测定HUVEC表面ICAM-1的表达,用实时定量PCR(RT-PCR)法检测ICAM-1的mRNA表达,用免疫荧光法检测核转录因子NF-κBp65蛋白的核转运。结果:隐丹参酮(3μM,1μM)可明显降低TNF-α诱导的HUVEC细胞表面粘附分子ICAM-1增多;明显抑制ICAM-1的基因表达;并对TNF-α诱导的HUVEC细胞核转录因子NF-κBp65蛋白的核转移有抑制作用。结论:隐丹参酮可剂量依赖性的抑制TNF-α诱导的HUVEC细胞粘附分子ICAM-1的基因表达以发挥抗动脉粥样硬化的作用,该作用的机制可能与其抑制转录因子NF-κBp65蛋白的核转运,从而抑制了NF-κB的激活有关。
Objective: To investigate the effect and mechanism of cryptotanshinone on the expression of ICAM-1 in human umbilical vein endothelial cells (HUVEC) induced by TNF-α. Methods: The model of HUVEC cell inflammation induced by TNF-α was established. Antioxidant pyrrolidine dithiocarbamate (PDTC) was used as a positive control. Immunocytochemistry was used to detect ICAM- 1 mRNA was detected by real-time quantitative PCR (RT-PCR), and nuclear translocation of NF-κBp65 was detected by immunofluorescence. Results: Cryptotanshinone (3μM, 1μM) significantly decreased the expression of ICAM-1 on the surface of HUVECs induced by TNF-α, significantly inhibited the gene expression of ICAM-1, and inhibited the expression of nuclear factor- κBp65 protein nuclear transfer inhibition. Conclusions: Cryptotanshinone can inhibit the gene expression of ICAM-1 induced by TNF-α in a dose-dependent manner in anti-atherosclerosis in HUVECs, which may be related to its inhibitory effect on the transcription factor NF-κBp65 Nuclear transport, thus inhibiting the activation of NF-κB.