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目的:测定河南地区汉族人群乙型肝炎e抗原阳性慢性乙型肝炎患者HLA-DRB1基因的多态性。方法:应用聚合酶链反应-序列特异性引物技术,对河南汉族38例乙型肝炎e抗原阳性慢性乙型肝炎患者及42例正常健康人(对照组)进行HLA-DRB1*1201/1202、*1301/1302、*1501/1502等位基因检测。结果:对照组HLA-DRB1*1301/1302等位基因频率高于乙型肝炎e抗原阳性慢性乙型肝炎组,差异有统计学意义(P<0.05);HLA-DRB1*1201/1202、*1501/1502等位基因频率2组间差异无统计学意义(P均>0.05)。结论:HLA-DRB1*1301/1302等位基因是河南汉族乙型肝炎e抗原阳性慢性乙型肝炎患者的一个保护基因。
Objective: To determine the polymorphism of HLA-DRB1 gene in patients with hepatitis B e antigen-positive chronic hepatitis B in Han nationality of Henan province. Methods: HLA-DRB1 * 1201/1202 and HLA-DRB1 were detected in 38 patients with hepatitis B e antigen-positive chronic hepatitis B and 42 healthy controls (control group) by using polymerase chain reaction-sequence specific primers. 1301/1302, * 1501/1502 allele test. Results: The frequency of allele HLA-DRB1 * 1301/1302 in control group was higher than that of hepatitis B e antigen positive chronic hepatitis B group (P <0.05); HLA-DRB1 * 1201/1202, * 1501 / 1502 allele frequency was no significant difference between the two groups (P all> 0.05). Conclusion: The HLA-DRB1 * 1301/1302 allele is a protective gene of hepatitis B e antigen-positive chronic hepatitis B patients in Henan Han nationality.