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目的探讨甲醛(FA)的DNA损伤机制。方法应用单细胞凝胶电泳技术研究不同浓度FA对V79细胞(中国仓鼠肺成纤维细胞)的DNA损伤程度,以及剂量依赖关系。结果单细胞凝胶电泳结果显示,在不同浓度FA(100～1 600μmol/L)彗星细胞拖尾率、尾长及头/尾比随着浓度的增加而减少,且差异具有统计学意义(P<0.01);特别是在1 600μmol/L时在图片中未见有彗星细胞出现,其显示的DNA损伤类型是DNA-蛋白质交联;但是FA的浓度与拖尾细胞率、尾长及头/尾比之间不存在线性关系。结论该研究结果认为,在体外培养条件下,一定浓度的FA能够引起细胞DNA损伤,主要表现为DNA-蛋白质交联。
Objective To investigate the DNA damage mechanism of formaldehyde (FA). Methods Single cell gel electrophoresis was used to study the DNA damage of V79 cells (Chinese hamster lung fibroblasts) with different concentrations of FA and the dose-dependent manner. Results The results of single cell gel electrophoresis showed that the tailing rate, tail length and head / tail ratio of comet cells with different concentrations of FA (100 ~ 1 600μmol / L) decreased with increasing concentration, and the difference was statistically significant (P <0.01). In particular, no comet cells appeared in the picture at 1 600 μmol / L. The type of DNA damage revealed was DNA-protein cross-linking. However, the concentration of FA and the ratio of tail cell length, tail length and head / There is no linear relationship between the tail ratios. Conclusions The results of this study suggest that certain concentrations of FA can cause DNA damage in vitro under in vitro culture conditions, mainly as DNA-protein cross-links.