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蛋白质二硫键异构酶(PDI)对蛋白的折叠和二硫键的形成起重要的作用.此外,PDI还执行许多其他的生物功能,是1个多功能酶.本文通过研究玉米中1个PDI基因的特征和表达,探讨它的功能作用.玉米中的PDI基因编码513个氨基酸.同源分析表明,该基因和水稻、小麦的PDI基因聚为一类,有很高的蛋白相似性.蛋白结构分析表明,该基因具有明显的PDI基因的结构特点,包括硫氧还蛋白活性位点(CGHC)以及内质网定位信号(KDEL).Northern杂交分析显示,该基因在发育种子的表达量高,同时受干旱、冷、ABA和盐等逆境胁迫诱导表达.PDI与GFP融合表达研究基因的亚细胞定位,表明该基因定位在除细胞膜外的细胞质和细胞器上.
Protein disulfide isomerase (PDI) plays an important role in protein folding and disulfide bond formation.In addition, PDI also performs many other biological functions and is a multifunctional enzyme.In this paper, PDI gene and its function.PDI gene in maize encoded 513 amino acids.Homologous analysis showed that the PDI gene of this gene was clustered into a group with high protein similarity. The structural analysis of the protein showed that the gene has obvious structural characteristics of PDI gene, including the thioredoxin active site (CGHC) and the endoplasmic reticulum localization signal (KDEL) .Northern hybridization analysis showed that the gene expression in developing seeds High, and at the same time it was induced by the stress of drought, cold, ABA and salt, etc. The subcellular localization of PDI and GFP fusion gene showed that the gene was localized on the cytoplasm and organelles except the cell membrane.