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应用免疫组织化学酶联亲和一步法标记库肯勃瘤组织中的雌激素受体（ＥＲ）和孕激素受体（ＰＲ）。结果表明：胃源性库肯勃瘤ＥＲ阳性率（６０％）明显高于大肠源性库肯勃瘤（２３．５％），统计学上有显著性意义（Ｐ＜０．０１）。胃癌比大肠癌更易转移到卵巢，胃源性库肯勃瘤生存期较大肠源性生存期要短。提出对双侧卵巢肿瘤患者应仔细对胃肠道等其它部位进行检查，以达到术前确诊，且对ＥＲ、ＰＲ阳性患者给予内分泌治疗，以求改善预后。
One-step immunohistochemistry was used to label the estrogen receptor (ER) and progesterone receptor (PR) in the tumor tissue of the Kukenberg tumor. The results showed that the positive rate of ER (60%) was significantly higher in gastric-derived Kukenberg tumors than in large-intestinal Kukenberg tumors (23.5%), which was statistically significant (P<0.01). Gastric cancer is more likely to metastasize to the ovary than colon cancer. Gastric-sourced Kukenberg tumors have a longer survival period and the intestinal survival time is shorter. It is proposed that patients with bilateral ovarian cancer should be carefully examined on other parts of the gastrointestinal tract to achieve preoperative diagnosis, and endocrine therapy should be given to patients with positive ER or PR, in order to improve the prognosis.