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目的:通过观察维吾尔族、汉族口腔鳞癌中细胞增殖和细胞凋亡之间的关系,探讨细胞凋亡在口腔鳞癌发生中的作用。方法:利用脱氧核糖核酸末端转移酶介导的dUTP缺口末端标记(TUNEL)技术及增殖细胞核抗原(PCNA)免疫组织化学染色,对69例(维36、汉33例)口腔鳞癌中的凋亡细胞和增殖细胞进行原位观察和比较。结果:维汉民族间增殖指数和凋亡细胞指数之间无显著差异(P>0.05),高分化口腔鳞癌与中低分化口腔鳞癌之间增殖指数及凋亡指数均有显著性差异(P<0.05),不同部位的口腔鳞癌其增殖指数无显著差异,而舌癌组凋亡指数高于唇癌组和牙龈癌组(P>0.05),增殖指数与凋亡指数在口腔鳞癌中呈负相关关系(r=-0.663)。结论:口腔鳞癌癌变过程不仅存在活跃的细胞增殖,而且存在细胞凋亡之异常,细胞增殖和细胞凋亡平衡失调在肿瘤发病中可能起重要作用。
OBJECTIVE: To investigate the relationship between cell proliferation and apoptosis in oral squamous cell carcinoma of Uygur and Han populations and to explore the role of apoptosis in the development of oral squamous cell carcinoma. METHODS: Using dUTP nick end labeling (TUNEL) mediated by DNA terminal transferase and immunohistochemical staining of proliferating cell nuclear antigen (PCNA), the apoptosis was detected in 69 cases (Vi 36, Han 33) of oral squamous cell carcinoma. Cells and proliferating cells were observed and compared in situ. RESULTS: There was no significant difference between the Uygur and Han nationality’s proliferation index and apoptotic cell index (P>0.05). There was a significant difference in proliferation index and apoptosis index between well-differentiated oral squamous cell carcinoma and moderately-differentiated oral squamous cell carcinoma. P<0.05), there was no significant difference in the proliferation index of oral squamous cell carcinoma at different sites, but the apoptotic index was higher in the tongue cancer group than in the lip cancer group and the gingival cancer group (P>0.05). The proliferation index and the apoptosis index were observed in oral squamous cell carcinoma. There was a negative correlation (r=-0.663). Conclusion: There are not only active cell proliferation but also abnormal apoptosis in oral squamous cell carcinoma. The imbalance of cell proliferation and apoptosis may play an important role in the pathogenesis of cancer.