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目的研究慢性阻塞性肺病(COPD)患者IL-6基因多态性在COPD发病机制的作用。方法提取30例COPD和30例健康者外周血DNA,扩增IL-6基因启动区2个单核苷酸多态性位点DNA序列并测序,测定IL-6血浓度。结果 COPD组与对照组血清IL-6差异有统计学意义(P<0.05);COPD组外周血IL-6基因启动区-572位点单核苷酸多态性(23.4%)与对照组(3.3%)比较,两组差异有统计学意义(P<0.05);比较COPD组中纯合突变GG型、杂合突变GC型及未出现突变三者之间的肺功能差异有统计学意义(P<0.05)。结论广东深圳汉族COPD患者IL-6基因启动区-572位点具有单核苷酸多态性,IL-6-572位点的G型等位基因可能与该地区汉族人群COPD发病有关,可能为COPD的易感基因。
Objective To investigate the role of IL-6 gene polymorphism in the pathogenesis of COPD in patients with chronic obstructive pulmonary disease (COPD). Methods DNA from 30 cases of COPD and 30 healthy controls were extracted and DNA sequence of IL-6 gene promoter region was amplified and sequenced. IL-6 blood concentration was measured. Results The level of IL-6 in COPD group and control group was significantly different (P <0.05). The SNP at -572 of IL-6 gene promoter in COPD group was significantly higher than that in control group (23.4% 3.3%), there was significant difference between the two groups (P <0.05). There was significant difference in lung function between homozygous mutant GG type, heterozygous mutant GC type and no mutation in COPD group P <0.05). CONCLUSION: SNP -572 in IL-6 gene promoter region of Han nationality in Shenzhen, Guangdong Province is single nucleotide polymorphism. G-type allele of IL-6-572 locus may be related to COPD incidence in Han population in this area, which may be COPD susceptibility genes.