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为了揭示无花果果实同化物韧皮部卸载机制,以玛斯义陶芬无花果5个时期果实为试材利用透射电子显微镜结合荧光染料示踪技术研究同化物韧皮部卸载路径的变化.无花果果实发育分3个时期,第1个快速生长期、缓慢生长期、第2个快速生长期,第2个生长期和第3个生长期之间为果实发育的转折期生长期之间.超微结构观察表明,无花果始熟期前筛分子伴胞复合体和周围薄壁细胞存在共质体联系,但始熟期后变为共质体隔离,同时在果实整个发育过程中薄壁细胞间始终存在大量胞间连丝.羧基荧光素(CF)引入表明,始熟期前荧光染料从韧皮部卸出到周围薄壁细胞中,在始熟期后CF被严格限制在韧皮部没有卸出到周围薄壁细胞.结果表明,无花果果实始熟期是同化物韧皮部卸载由共质体向质外体转变的转折期,但同时韧皮部后运输始终为共质体途径.
In order to reveal the unloading mechanism of the fig fruit fruit assimilate phloem, the changes of unloader path in the phloem of the assimilate were studied by transmission electron microscopy and fluorescent dye tracing technique using the fruits of Mastilline fig as test material for 5 periods.The growth of fig fruit was divided into three periods , The first rapid growth period, the slow growth period, the second rapid growth period, the second growth period and the third growth period between the fruit development of the transitional growth period ultrastructural observations show that the fig Before maturity, there was a symbiotic relationship between the SCC complex and the surrounding parenchyma cells, but it became a cytosol after the initial ripening stage. At the same time, there were always a large number of interstitial cells The introduction of carboxyfluorescein (CF) indicated that the fluorescent dye was discharged from the phloem to the surrounding parenchyma cells prior to the initial ripening stage, and CF was severely restricted in the phloem after initial ripening and was not released to surrounding parenchymal cells. , Fig fruit ripening stage is the turning point of the transition from apoplast to apoplast in the phloem of the assimilate, but at the same time, the phloem transport is always the way of the plastid.