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为筛选适宜配制华山松大小蠹(Dendroctonus armandi)引诱剂的有效化学成分,用触角电位仪和Y型嗅觉仪测定了华山松大小蠹雌、雄虫对华山松挥发物主要成分的触角电生理(EAG)和行为反应。结果表明:在0.01、0.1、1、10、100μg5种刺激剂量下,7种化合物在特定浓度下均有明确的EAG反应;雌、雄虫对月桂烯、(+)-3-蒈烯、β-石竹烯3种化合物及雄虫对(+)-α-蒎烯、柠檬烯的EAG相对电位的最大值均出现在10μg刺激条件下;在10μg刺激剂量条件下,雌虫对(+)-α-蒎烯、β-蒎烯和(+)-3-蒈烯有明显的正趋向反应,正趋向率均高于72%且显著高于雄成虫对3种挥发物的正趋向率;在1μg刺激下,只有雄虫对(+)-3-蒈烯有一定正趋向反应;而在100μg条件下,(-)-α-蒎烯对雌、雄虫均有一定驱避作用。该研究结果可为开发华山松大小蠹林间引诱剂提供借鉴。
In order to screen the effective chemical constituents suitable for the attractant of Dendroctonus armandi, the main components of the volatiles of Pinus armandi, the female and male of Pinus armandi, were measured by the antennae and Y-olfactometer. EAG) and behavioral responses. The results showed that all of the seven compounds showed definite EAG response under the five concentrations of 0.01, 0.1, 1, 10 and 100 μg. The activity of mycotoxins, mycophenolate, β - The maximal EAG relative potentials of (+) - α-pinene and limonene in the three compounds of caryophyllene and male were all observed at 10μg stimulus. Under the stimulation dose of 10μg, - pinene, β-pinene and (+) - 3-carene have a positive trend reaction, the positive trend rate was higher than 72% and significantly higher than the male adults on the three volatile positive trend; 1μg stimulus , Only the male had a positive trend towards (+) - 3-carene, while (-) - α-pinene had a certain repellent effect on female and male at 100μg. The results of this study can provide reference for the development of Pinus armandii forest lure.