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目的了解北京市部分男男同性恋(men who have sex with men,MSM)人群HIV-1的分子流行情况,监测该地区人群HIV毒株的最新流行情况。方法提取17例男男同性恋者HIV-1抗体阳性样本全血基因组DNA,直接进行巢式PCR扩增HIVenv基因C2-V5区及gag基因P17-P24区,对PCR产物进行核苷酸序列测定和分析,确定基因亚型。结果17例患者中,共存在CRF01-AE、B亚型和CRF07-BC3种亚型,其中CRF01-AE9例(52.94%),B亚型5例(29.41%),CRF07-BC3例(17.65%)。结论小样本量的流行病学调查显示,北京地区部分男男同性恋人群中主要存在CRF01-AE、B和CRF07-BC3种亚型;CRF01-AE已取代B亚型而成为北京市MSM人群中HIV主要流行亚型,CRF07-BC重组亚型在北京市MSM人群中增长迅速。
Objective To understand the molecular epidemic of HIV-1 in men who have sex with men (MSM) in Beijing and to monitor the latest epidemic situation of HIV in this region. Methods Genomic DNA of 17 HIV-1 positive samples of gay men was extracted from the whole blood samples by direct nested PCR to amplify the C2-V5 region of HIVenv gene and P17-P24 region of gag gene. The PCR products were sequenced and Analysis, to determine the genotypes. Results Among the 17 patients, there were 3 subtypes of CRF01-AE, B and CRF07-BC, including 9 cases of CRF01-AE (52.94%), 5 cases of B subtype (29.41%) and 3 cases of CRF07-BC (17.65% ). Conclusions Epidemiological survey of small sample size shows that CRF01-AE, B and CRF07-BC3 subtypes are mainly found in some gay men in Beijing. CRF01-AE has replaced subtype B as a marker of HIV among MSM in Beijing The major epidemic subtype, CRF07-BC recombinant subtype in Beijing MSM population growing rapidly.