采用聚丙烯酰胺凝胶垂直平板电泳技术，对选自中国芝麻资源７个生态区的３６个地方品种进行了酯酶、过氧化物酶、苹果酸脱氢酶、６—磷酸葡萄糖脱氢酶、淀粉酶５种同工酶分析，仅酯酶酶谱表现出品种间和品种内的多样性差异。对芝麻核心种质和非核心种质中各９个品种的１０８０个植株酯酶同工酶分析，结果表明在迁移率Ｒｆ０．４５～０．６７间检测到８种酶谱类型。通过对核心种质与非核心种质的酯酶酶谱类型和酶带分布频率、品种多样性指数比较及聚类分析，表明核心种质良好地代表了非核心种质。 Thirty-six landraces selected from 7 ecotopes of China sesame resources were subjected to polyacrylamide gels vertical plate electrophoresis. Esterase, peroxidase, malate dehydrogenase, 6-phosphoglucose dehydrogenase, Five isozymes of amylase were analyzed. Only the esterase zymogram showed diversity within and among cultivars. The analysis of esterase isozymes of 1,080 plants in 9 varieties of core sesame and non-core germplasm of sesame showed that there were 8 types of zymograms in the Rf 0.45-0.67. Through the comparison of the types of esterase and the bands of the enzyme bands, the diversity indexes of the varieties and the cluster analysis of the core and non-core germplasms, it shows that the core germplasm represents the non-core germplasm well.