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目的：观察PDGF－AB对体外培养的成纤维细胞超微结构的影响，探讨PDGF－AB促进创面愈合的机制．方法：取第3～6代培养的成纤维细胞，用0μg／L，12．5μg／L，50μg／L的PDGF－AB分别作用48h，然后用扫描电镜和透射电镜观察．结果：PDGF－AB明显增加成纤维细胞表面突起和纤维样物质，以及增强成纤维细胞的合成功能．结论：PDGF－AB可能通过激活、趋化成纤维细胞、增强细胞合成功能来促进创面愈合．
OBJECTIVE: To observe the effect of PDGF-AB on the ultrastructure of cultured fibroblasts in vitro and to explore the mechanism of PDGF-AB promoting wound healing. Methods: Fibroblasts from the 3rd to 6th passage were harvested for 48h with 0μg / L, 12.5μg / L and 50μg / L of PDGF-AB respectively, and then observed by scanning electron microscopy and transmission electron microscopy. Results: PDGF-AB significantly increased the surface processes of fibroblasts and fibrous material, and enhanced the synthesis of fibroblasts. Conclusion: PDGF-AB may promote wound healing by activating, chemotacticizing fibroblasts and enhancing cell synthesis.