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冬大麦(Hordeum vulgare L.)栽培品种 GerbeI 的叶片用大麦黄花叶病病毒汁液接种。检验了接种液的不同附加液。而植株汁液单用水稀释导致43%的感病率,添加硫酸钠(80%)和磷酸钾缓冲液(89%)以后基本上提高了感病植株的比例。借助重复接种进一步提高了侵染性,硫酸钠产生96%的侵染,磷酸钾为100%的侵染效果。讨论了进一步研究和应用这个技术的实用性。
The leaves of the GerbeI cultivar Hordeum vulgare L. were inoculated with the Yellow Mosaic Virus virus solution. Different adjuncts of the inoculum were tested. However, dilution of the plant juice with water alone resulted in a 43% susceptibility, with the proportion of susceptible plants substantially increased after the addition of sodium sulfate (80%) and potassium phosphate buffer (89%). Infectivity was further increased by repeated inoculations, with 96% infection with sodium sulfate and 100% infection with potassium phosphate. Discusses the practicality of further research and application of this technology.