目前常规的蒸汽接种法,有的是设法使小空间始终有大量的蒸汽,造成一种无菌环境,有的是使蒸汽通过灭菌药液,使蒸汽内含有杀菌剂,达到无菌操作的目的.在所有接种方法中,都有一个用酒精灯对接种工具和接种口灭菌的过程,酒精灯由于火焰有限,在蒸汽内会熄灭,因此只有接种工具和接种口离开蒸汽,才能用酒精灯灭菌,这样就增加了污染的可能性.笔者经多 At present, the conventional steam inoculation method is to try to make a small space always have a lot of steam, resulting in a sterile environment, and some is to pass steam sterilization liquid, the steam contains fungicides for the purpose of aseptic operation.In all Inoculation methods, there is an alcohol lamp vaccination tools and inoculation sterilization process, the alcohol lamp due to the flame is limited, in the steam will be extinguished, so only vaccination tools and inoculation to leave the steam can be sterilized with alcohol lamp, This will increase the possibility of pollution