内毒素(脂多糖,LPS)为革兰阴性细菌外膜组成部分,能引发一系列毒性介质释放,诱导巨噬细胞释放大量细胞因子(如肿瘤坏死因子-α,TNF-α)等。LPS分子有三个主要区:1)最外层的O-抗原多糖区,每种革兰阴性细菌有其独特的多糖区;2)核心多糖区,在多种革兰阴性细菌是相似的;3)毒性脂质A区。实验应用抗-大肠杆菌0111:B4的O-抗原多糖区的两种单克隆抗体(mAb)即8G9(IgG_3)和3D10(IgM),和抗-LPS的DCLA交叉反应性mAb1B6(IgG_(2a))。制做缺少IgG分子的Fc部分 Endotoxin (LPS), a component of the outer membrane of Gram-negative bacteria, can trigger a series of release of toxic mediators and induce macrophages to release a large number of cytokines (such as tumor necrosis factor-α and TNF-α). The LPS molecule has three major regions: 1) the outermost O-glycan region, each of which has its own unique polysaccharide region; 2) the core polysaccharide region, which is similar to many Gram-negative bacteria; 3 Toxic lipid A zone. Two monoclonal antibodies (mAbs), namely 8G9 (IgG_3) and 3D10 (IgM) against anti-E. coli 0111: B4 O-antigen polysaccharide region and DCLA cross-reactive mAb 1B6 (IgG_ (2a) ). The Fc portion lacking the IgG molecule was made