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将来自加拿大艾伯塔的5种十二指肠贾第虫虫株:H8,PB1,OAS1,DOGD3和MR4(其宿主分别为人、海狸、羊、狗和麝鼠),按常规方法进行培养。分别提取其基因组DNA,用限制性内切酶Hinf I切开,以单股噬菌体M13 DNA为探针做指纹图。结果表明,5种虫株的一些较小的杂交带相似,但1～4kb的杂交带显示出差异,尤以OAS1株多一条2kb左右的带。5种虫株的主带在1.5和3kb处变异明显。每种虫株均表现出其独特的DNA指纹图型。由于这些虫株在以前的研究中曾显示抗原性和DNA图型十分相似,因而有学者推断其在遗传学上有密
Five species of Giardia lamblia: H8, PB1, OAS1, DOGD3 and MR4 from Alberta, Canada, whose hosts are human, beaver, sheep, dog and muskrat, respectively, were cultured according to a conventional method. Genomic DNA was extracted and cut with restriction endonuclease Hinf I. Fingerprinting was performed using single-stranded phage M13 DNA as a probe. The results showed that some of the five insect isolates had similar bands, but 1 ~ 4 kb hybrids showed differences, especially about 2 kb in OAS1 strains. The main band of 5 kinds of insect strains mutated significantly at 1.5 and 3kb. Each strain shows its unique DNA fingerprinting pattern. Since these strains have shown antigenicity and DNA patterns to be similar in previous studies, some scholars have deduced that they are genetically closely related