本文介绍用K562作为靶细胞测定NK活性和临床应用,结果如下。 方法:取静脉血5毫升,用肝素抗凝,采用连续密度梯度分离法,分离出单个核细胞作为效应细胞计数,测定活性后备用。 用~(51)铬标记K562,Hela细胞,作为靶细胞备用。靶效比例,K562为1:10,37℃孵育4小时;而Hela细胞为1:20,孵育18小时。孵育后离心沉淀, This article describes the use of K562 as a target cell determination of NK activity and clinical application, the results are as follows. Methods: Take 5 ml of venous blood and anticoagulant with heparin. The mononuclear cells were counted as effector cells by continuous density gradient method and the activity was reserved. K562 and Hela cells were labeled with ~ (51) Cr and used as target cells. Targeted ratio, K562 was 1:10, 37 ° C for 4 hours; Hela cells 1:20, incubated for 18 hours. After incubation, pelleted,