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采用氯仿熏蒸法、稀释平板法和磷脂脂肪酸(phospholipidfattyacid,PLFA)方法,分析了常绿阔叶林转变成杉木人工林后土壤微生物种群数量和群落结构的变化特征.结果表明:常绿阔叶林转变为杉木人工林后,林地土壤的微生物生物量碳、可培养细菌和放线菌数降低.杉木人工林地总PLFAs、细菌PLFAs、真菌PLFAs比常绿阔叶林分别降低了49.4%、52.4%和46.6%,革兰氏阳性和阴性细菌PLFAs远低于常绿阔叶林.杉木人工林根际土壤微生物生物量碳、可培养细菌和放线菌数显著高于杉木人工林林地土壤,根际土壤中总PLFAs、细菌PLFAs、革兰氏阳性和阴性细菌PLFAs的含量也高于林地土壤,但真菌PLFAs和细菌PLFAs之比却低于林地土壤.对土壤微生物群落结构进行主成分分析发现,第1主成分和第2主成分共解释了土壤微生物群落结构变异的78.2%.表明常绿阔叶林与杉木人工林土壤的微生物群落结构间存在差异.
The changes of population and community structure of soil microbe after the conversion of evergreen broad-leaved forest into Chinese fir plantation were analyzed by chloroform fumigation, dilution plate method and phospholipid fatty acid (PLFA) method. The results showed that: After conversion to Cunninghamia lanceolata plantation, the soil microbial biomass carbon, culturable bacteria and actinomycetes decreased, and the total PLFAs, bacterial PLFAs and fungi PLFAs decreased by 49.4% and 52.4% respectively compared with the evergreen broad-leaved forest, And 46.6% respectively, while the gram positive and negative bacteria PLFAs were much lower than that of the evergreen broad-leaved forest.The microbial biomass carbon, culturable bacteria and actinomycetes in rhizosphere soil of Chinese fir plantation were significantly higher than those in forest plantation and root The content of total PLFAs, bacterial PLFAs, Gram-positive and negative bacteria PLFAs in the soils was also higher than that of woodland soil, but the ratio of fungi PLFAs and bacteria PLFAs was lower than that of woodland soil.The principal component analysis of soil microbial community structure found that, The first principal component and the second principal component explained a total of 78.2% of the variance of soil microbial community structure, indicating that between the evergreen broadleaved forest and the Cunninghamia lanceolata plantation soil microbial community structure Differences.