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目的:建立火炭母药材的HPLC指纹图谱分析方法,为火炭母药材质量评价提供参考依据。方法:色谱柱:Platisil ODS C18(250mn×4.6mm,5μm);流动相为乙腈-0.05%磷酸溶液,梯度洗脱进行色谱分离;洗脱时间为60min,体积流量:1.0mL.min-1,检测波长:360nm。结果:确定17个共有色谱峰为特征峰构成火炭母药材HPLC指纹图谱,相似度评价结果表明,各产地火炭母药材相似度均在0.90以上。结论:所用方法准确可靠,所得指纹图谱共有模式可作为火炭母药材质量控制的依据。
OBJECTIVE: To establish a HPLC fingerprinting method for detecting the medicinal materials of firewood, and to provide a reference for the quality evaluation of the medicinal materials of firewood. Methods: The chromatographic column was Platisil ODS C18 (250mn × 4.6mm, 5μm). The mobile phase consisted of acetonitrile-0.05% phosphoric acid solution and eluted with gradient elution. The elution time was 60min, the volume flow rate was 1.0mL.min-1, Detection wavelength: 360nm. Results: Seventeen common chromatographic peaks were identified as the HPLC fingerprints of Fragrans, and the similarity evaluation indicated that the similarity of Fragrant Medicinal Herbs in all producing areas was above 0.90. Conclusion: The method used is accurate and reliable. The common pattern of the obtained fingerprints can be used as the basis for the quality control of the medicinal materials of firewood.