在杀线虫剂活性测定中,面临一大难题就是要快速、准确地判断被试线虫的死活,这是提高工作效率和测试准确性的重要环节。线虫死活的鉴别一般采用体态法、即死的虫体多呈僵直状态,而活的体态是几度弯曲,一般盘卷和蠕动,但对休眠态、体形膨大的雌虫和卵不适用。此外,线虫不动不等于死。因此,在杀线虫剂测定中,除用击倒率表示药效外,还需建立一种快速,准确地判断线虫死活的方法。为此,我们摸索了几种生物染料对 In the determination of nematicide activity, a major challenge is to quickly and accurately determine the test nematode’s life and death, which is to improve work efficiency and test accuracy is an important part. Nematode identification of the general use of body and death method of life and death, that dead body mostly stiff state, and live in a state of several degrees of bending, the general coil and peristalsis, but the dormant state, the shape of enlarged females and eggs do not apply. In addition, nematodes do not move to death. Therefore, nematicide determination, in addition to using the knockdown rate that efficacy, but also need to establish a quick and accurate method to determine the survival and death of nematodes. To this end, we explored several biological dye pairs