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本文用剂量-效应关系比较苯妥英和可的松对小鼠致畸作用,并探讨两者所致腭裂的机理。实验用CF-1小白鼠,体重25～35g,交配前雌雄分笼两周以上,室内光照每天12小时(上午7:00至下午7:00),室温22～26℃。可的松溶于盐水供肌内注射,剂量分为25、50、70、100和125mg/kg5组。苯妥英溶于丙二醇供皮下注射,分50、70、80和90mg/kg4组。对照动物分为不处理、盐水肌内注射和丙二醇皮下注射3组。所有动物均于妊娠第11～14天给药,妊娠18天解剖查胎仔
In this paper, dose-effect relationship between phenytoin and cortisone teratogenic effect on mice, and explore the mechanism of cleft palate caused by both. Experimental CF-1 mice, body weight 25 ~ 35g, before mating male and female cage more than two weeks, indoor lighting 12 hours a day (7:00 am to 7:00 pm), room temperature 22 ~ 26 ℃. Cortisone was dissolved in saline for intramuscular injection in doses of 25, 50, 70, 100 and 125 mg / kg of 5 groups. Phenytoin dissolved in propylene glycol for subcutaneous injection, divided into 50,70,80 and 90mg / kg4 group. The control animals were divided into three groups: untreated, saline intramuscular injection and propylene glycol subcutaneous injection. All animals were administered on the 11th to 14th days of gestation, and fetuses were dissected on the 18th day of gestation