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G蛋白在信号转导中起着重要的作用,许多研究表明Gq蛋白还具有其他功能。为了明确Gqα在信号转导中的具体作用,本研究以Gqα为诱饵,利用酵母双杂交技术对棉铃虫Helicoverpa armigera(Hbner)触角cDNA文库进行筛选。结果表明:信息素结合蛋白(PBP)、3-磷酸甘油醛脱氢酶(GAPDH)、核糖体蛋白S20、细胞色素氧化酶亚基Ⅰ、促咽侧体神经肽前体(AT2)、翻译控制的肿瘤蛋白(TCTP)、体液凝集素前多肽原和糖基化天冬酰胺酶等10多种蛋白与Gqα发生结合。据此推断:Gqα可能在信号转导过程中发挥多种作用;在多种功能中,Gqα最可能参与嗅觉信号传导中的信息素识别。这些研究结果可能为将来建立棉铃虫完整的信号网络提供基础。
G protein plays an important role in signal transduction. Many researches show that Gq protein has other functions. In order to clarify the specific role of Gqα in signal transduction, this study used Gqα as bait to screen the Helicoverpa armigera (Hbner) antennae cDNA library by yeast two-hybrid technique. The results showed that the expression of pheromone binding protein (PBP), glyceraldehyde 3 - phosphate dehydrogenase (GAPDH), ribosomal protein S20, cytochrome oxidase subunit Ⅰ, preophoric neuropeptide precursor (AT2) Tumor protein (TCTP), pre-humoral agglutinin propeptide and glycosylated asparaginase and other 10 kinds of proteins and Gqα binding occurs. It is concluded that Gqα may play a variety of roles in signal transduction. Among many functions, Gqα is most likely involved in pheromone recognition in olfactory signaling. These findings may provide a basis for establishing a complete signaling network for H. armigera in the future.