论文部分内容阅读
目的应用彗星试验和微核试验研究铅致职业接触人群遗传损伤效应。方法3家蓄电池厂的187名职业接触铅工人作为铅接触组,同时选择某镇无铅和其他职业危害接触史的村民179人作为对照组,进行彗星试验和微核试验。结果铅接触组彗星率、彗尾长度和微核细胞率均大于对照组,且差异有统计学意义(P<0.001);随着血铅或尿铅水平的增高,彗星率有增高趋势,微核细胞率仅在血铅>1.45μmol/L组或尿铅>0.58μmol/L组与对照组比较差异有统计学意义(P<0.001)。结论铅可引起职业接触人群遗传损伤,彗星试验可作为铅职业接触人群遗传损伤效应的检测方法。
Objective To study the effects of lead exposure on genetic damage in lead-exposed workers by using comet assay and micronucleus test. Methods A total of 187 occupational exposure lead workers in three battery factories were selected as the lead exposure group. 179 villagers from a certain town who had no exposure to lead and other occupational hazards were selected as the control group for comet assay and micronucleus test. Results The comet rate, the length of tail and the rate of micronucleated cells in lead exposure group were significantly higher than those in control group (P <0.001). The comet rate increased with the increase of blood lead or urine lead level. The rate of nucleated cells was only significantly different from that of the control group (P <0.001), only in the group of 1.45 μmol / L of blood lead or 0.58 μmol / L of lead in urine. Conclusion Lead may cause genetic damage to occupationally exposed people and comet assay may be used as a detection method for the genetic damage effect of lead exposed workers.