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目的:建立肺炎克雷伯菌的基因扩增多态性DNA分型方法,了解产ESBLs肺炎克雷伯菌院内感染的分子流行情况。方法:收集临床各病区送检的产ESBLs肺炎克雷伯菌感染标本29份。采用随机扩增DNA多态性方法对产ES-BLs肺炎克雷伯菌进行基因分型。利用Phylip3.67聚类分析软件对RAPD扩增条带结果进行聚类分析。结果:RAPD扩增的指纹图谱清晰,带型稳定,多态性丰富。29株不同来源的菌株可大致分为三种亲缘关系。结论:随机扩增多态性DNA技术可以用于产ESBLs肺炎克雷伯菌基因多态性分型研究;产ESBLs肺炎克雷伯菌基因多态性分布可能与菌株的来源有关。
OBJECTIVE: To establish a method for genotyping Klebsiella pneumoniae by genotyping DNA polymorphism and to understand the molecular epidemic situation of nosocomial infection of ESBLs-producing Klebsiella pneumoniae. Methods: 29 clinical isolates of ESBLs-producing Klebsiella pneumoniae were collected for clinical examination. Genotyping of ESBLs producing Klebsiella pneumoniae by random amplified polymorphic DNA. The clustering analysis of RAPD amplified bands was carried out by using Phylip3.67 cluster analysis software. Results: The fingerprinting of RAPD amplification was clear, the band pattern was stable and the polymorphism was abundant. 29 strains from different sources can be roughly divided into three kinship. Conclusion: The random amplified polymorphic DNA (PCR) technique can be used to genotype the Klebsiella pneumoniae ESBLs-producing isolates. The genetic distribution of Klebsiella pneumoniae producing ESBLs may be related to the origin of the strains.