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目的探讨4-(甲基亚硝氨基)-1-(3-吡啶基)-1-丁酮(NNK)对中国仓鼠肺细胞V79的体外毒性作用。方法采用MTT法检测不同浓度NNK(0.1、0.2、0.3、0.4和0.5 mg/ml)对V79细胞存活率的影响。流式细胞仪检测不同浓度NNK体外作用24 h对V79细胞凋亡率和周期分布的影响。结果 NNK对V79细胞具有明显的抑制体外增殖作用。MTT结果显示抑制呈时间和浓度的依赖性(P<0.05)。流式细胞仪检测显示,各实验组细胞经NNK作用24 h后,细胞凋亡率分别为27.20%±1.26%、35.91%±1.98%、42.68%±2.27%、49.55%±2.81%和63.18%±3.52%,与对照组比较差异有统计学意义(P<0.05)。细胞周期发生G0/G1阻滞。结论 NNK对V79细胞有明显的增殖抑制作用,其机制可能与诱导和促进细胞凋亡相关。
Objective To investigate the in vitro cytotoxicity of 4- (methylnitrosamino) -1- (3-pyridyl) -1-butanone (NNK) on Chinese hamster lung V79 cells. Methods The effects of different concentrations of NNK (0.1, 0.2, 0.3, 0.4 and 0.5 mg / ml) on the viability of V79 cells were detected by MTT assay. Effect of different concentrations of NNK in vitro on the apoptosis rate and the period distribution of V79 cells 24 h by flow cytometry. Results NNK could significantly inhibit the proliferation of V79 cells in vitro. The MTT results showed that the inhibition was time-dependent and concentration-dependent (P <0.05). Flow cytometry showed that the apoptotic rates of cells in each experimental group were 27.20% ± 1.26%, 35.91% ± 1.98%, 42.68% ± 2.27%, 49.55% ± 2.81% and 63.18% respectively, ± 3.52%, compared with the control group, the difference was statistically significant (P <0.05). Cell cycle G0 / G1 block. Conclusion NNK can significantly inhibit the proliferation of V79 cells, and its mechanism may be related to the induction and promotion of apoptosis.