目的为了研究哈尔滨市庚型肝炎病毒（ＨＧＶ）非结构（ＮＳ５）区基因结构特征。方法对阳性标本进行了庚型肝炎病毒ＮＳ５区１８６核苷酸的基因扩增、分子克隆和序列分析，并利用基因分析软件处理结果。结果分离出的２株ＨＧＶＮＳ５区基因序列之间同源性为９５２％，而与国外报道的３株ＨＧＶ序列比较同源性在８４４％～９２４％，变异较大。结论提示ＨＧＶ有不同的基因型，这有待于对各区基因序列进行全面检测，综合判断之后才能确定。分离出的阳性株为从１９８４年肝炎患者血中分离，肯定了我市在８０年代就有庚型肝炎病毒感染存在 Objective To study the structural features of the non-structural (NS5) region of hepatitis G virus (HGV) in Harbin. Methods Positive samples were amplified by 186 nucleotides of GST region of Hepatitis G virus, molecular cloned and sequenced, and the results were analyzed by gene analysis software. Results The homology between the two HGVNS5 regions was 952%, while the homology was 844% ~ 924% with the three HGV sequences reported abroad. The conclusion suggests that there are different genotypes of HGV, which needs to be comprehensively tested on the gene sequences of each region, and can be determined after comprehensive judgment. The positive isolates were isolated from the blood of hepatitis patients in 1984, confirming the existence of hepatitis G virus infection in our city in the 1980s