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目的 了解新疆妇女人乳头瘤病毒 16型 (HPV 16)的感染状况。方法 用PCR及荧光定量PCR(FQ -PCR)方法检测妇科门诊普通患者宫颈脱落细胞及分泌物中人乳头瘤病毒 16型E6基因 (HPV16E6) ,以普通门诊患者宫颈脱落细胞及分泌物DNA作为样本 ,以人乳头瘤病毒 (HPV16E6)作为扩增的靶基因 ,同时以人 β -actin基因片段作为细胞内参照 ,借助于两对引物 ,两个特异的荧光探针 ,用荧光定量PCR(FQ -PCR)方法对这两个片段进行扩增 ,得到单位细胞HPV16E6的相对含量 ;同时进行定性PCR检测。结果 宫颈脱落细胞标本 15 9例中 ,β -actin阳性 15 4例 ;HPV 16E6阳性共 12例 ,阳性率为 7.8%。PCR与FQ -PCR结果基本一致 ,但FQ -PCR更敏感。结论 建立的FQ -PCR检测宫颈脱落细胞内HPV 16E6基因的方法 ,能反映单位细胞内病毒的复制情况 ,可用于性传播感染 (STI)及宫颈癌的筛查
Objective To understand the infection status of human papillomavirus type 16 (HPV 16) in Xinjiang women. Methods Human papillomavirus type 16 E6 gene (HPV16E6) was detected in cervical exfoliative cells and secretions of gynecological outpatients by PCR and real-time fluorescence quantitative PCR (FQ-PCR). Samples of cervical exfoliative cells and secretions DNA were collected from common outpatients , Human papillomavirus (HPV16E6) was used as the target gene for amplification. At the same time, human β-actin gene fragment was used as an internal reference. Two specific primers were used to detect the expression of human β-actin gene. Fluorescent quantitative PCR (FQ - PCR) method to amplify these two fragments to get the relative content of HPV16E6 cells; at the same time qualitative PCR detection. Results 15 out of 15 samples of cervical exfoliated cells, β-actin positive 15 4 cases; HPV 16E6 positive in 12 cases, the positive rate was 7.8%. PCR and FQ-PCR results are basically the same, but FQ-PCR more sensitive. Conclusion The established method of FQ-PCR to detect HPV 16E6 gene in cervical exfoliated cells can reflect the replication of virus in unit cell and can be used in STI and screening of cervical cancer