目的:探讨冬凌草甲素单用及联合柔红霉素对急性T淋巴细胞白血病jurkat细胞增殖抑制的影响。方法:采用CCK-8法检测单用冬凌草甲素、柔红霉素对jurkat细胞的抑制作用,及两种药物联合应用对jurkat细胞的抑制作用;采用Giemsa法检测冬凌草甲素作用于jurkat细胞48h的凋亡形态学变化;采用流式细胞仪检测单用冬凌草甲素对jur-kat细胞的凋亡率及两药物联合时的凋亡率。结果:单用不同浓度的冬凌草甲素(1、2、4、8和16μg/mL)对jurkat细胞具有增殖抑制作用,且呈时间-浓度依赖性,其中干预48h后的抑制率分别为(10.80±1.58)%、(32.32±7.83)%、(42.27±4.43)%、(55.07±1.65)%和(70.36±4.11)%;当联合小剂量柔红霉素(0.04μg/mL)时对jurkat细胞的抑制作用显著增加。Giemsa染色后,随着药物浓度的增加,镜下可观察到胞体皱缩、染色质浓集和凋亡小体等形态学变化。不同浓度的冬凌草甲素干预jurkat细胞48h后的凋亡率分别为(7.74±0.96)%、(13.26±1.49)%、(17.42±1.24)%、(25.13±2.12)%和(29.07±0.59)%,呈浓度依赖性增加;单用柔红霉素(0.04μg/mL)干预jurkat细胞48h后的凋亡率为(18.19±1.33)%;当冬凌草甲素(4μg/mL)联合柔红霉素(0.04μg/mL)干预jurkat细胞时,其凋亡率为(51.06±2.25)%,与单用两种药物相比差异有统计学意义,P<0.05。结论:冬凌草甲素能够抑制jurkat细胞增殖和诱导凋亡,联合柔红霉素对jurkat细胞的增殖抑制作用显著增强,具有协同抑制效应。 Objective: To investigate the effects of oridonin alone and in combination with daunorubicin on the proliferation of acute lymphoblastic leukemia jurkat cells. Methods: The inhibition of jurkat cells with oridonin and daunorubicin by CCK-8 alone and the combination of two drugs on jurkat cells were detected by CCK-8 assay. The effect of oridonin Morphological changes of apoptosis in jurkat cells 48h were observed. Flow cytometry was used to detect the apoptosis rate of jur-kat cells with oridonin alone and the apoptosis rate of the two drugs. Results: The inhibitory rates of oridonin (1, 2, 4, 8 and 16 μg / mL) alone on jurkat cells were in a time-and concentration-dependent manner, and the inhibitory rates after 48 h intervention were (10.80 ± 1.58)%, (32.32 ± 7.83)%, (42.27 ± 4.43)%, (55.07 ± 1.65)% and (70.36 ± 4.11)%, respectively; when combined with low dose of daunorubicin Inhibition of jurkat cells was significantly increased. Giemsa staining, with the increase of drug concentration, microscopic observation of cell shrinkage, chromatin condensation and apoptotic bodies and other morphological changes. The apoptosis rates of jurkat cells treated with different concentrations of oridonin for 48 hours were (7.74 ± 0.96)%, (13.26 ± 1.49)%, (17.42 ± 1.24)%, (25.13 ± 2.12)% and (29.07 ± 0.54%) in a dose-dependent manner. The apoptosis rate of jurkat cells treated with daunorubicin (0.04μg / mL) for 48h was (18.19 ± 1.33)%, while that of oridonin The apoptosis rate of jurkat cells treated with daunorubicin (0.04μg / mL) was (51.06 ± 2.25)%, which was significantly different from that of the two drugs alone (P <0.05). Conclusion: Rubescensine A can inhibit the proliferation and induce apoptosis of jurkat cells. The combination of daunorubicin and jurkat cells can significantly inhibit the proliferation of jurkat cells with synergistic inhibitory effect.