组织分离是利用菌体幼嫩的活组织,在适宜的培养基和生长条件下,由于实体发育阶段回复到菌丝生长阶段,变成没有组织化的菌丝体,是获得纯菌种最简便的一种方法.在生产上,由于灵芝的担孢子不易萌发,故常采用组织分离法获得菌种,为了高效、快速、准确地将这一方法应用于实践中,我们对代料栽培灵芝的组织分离进行了较系统的研究,以期获得一种安全有效的方法. Tissue separation is the use of cells of young living tissue, in the appropriate medium and growth conditions, due to the physical development stage back to the mycelial growth stage, into a non-organized mycelium is the most simple and easy access to pure species In the production, due to the fungus spores of ganoderma lucidum is not easy to germinate, it is often used to obtain strains of tissue separation method, in order to efficient, fast and accurate application of this method in practice, we reorganized the organization of Ganoderma lucidum Separation of a more systematic study, in order to obtain a safe and effective method.