人工合成的寡聚脱氧核苷酸是研究生物体系内基因抑制的理想工具。能与DNA双链结合形成三股螺旋的寡聚脱氧核苷酸具有特殊意义,因为它是与基因本身结合,而不是象在及意义策略中与基因的产物mRNA结合。将能形成三股螺旋的寡核苷酸与修饰DNA的试剂偶联后可用于引起靶DNA以高度序列特异性的方式发生修饰。我们最近设计了补骨脂素-寡核苷酸的结合物,它一旦通过形成三股螺旋与双链DNA结合后,即能在体外与经过紫外线照射的DNA的二条链发生交叉连接。一种补骨脂素-寡核苷酸的结合物被导向白介素2受体α亚 Synthetic oligodeoxynucleotides are ideal tools for studying gene repression in biological systems. It is of particular interest that an oligodeoxynucleotide that forms a triple helix that binds to a double strand of DNA because it binds to the gene itself rather than to the product mRNA of the gene in an as and sense strategy. Coupling the triple helix-forming oligonucleotide to a reagent that modifies the DNA can be used to cause the target DNA to be modified in a highly sequence-specific manner. We recently designed a psoralen-oligonucleotide conjugate that, once bound to double-stranded DNA by forming triple helixes, cross-links with both strands of UV-irradiated DNA in vitro. A psoralen-oligonucleotide conjugate is directed to the interleukin 2 receptor alpha subunit