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目的探讨亚硝基谷胱甘肽(GSNO)对冰冻血小板聚集、一氧化氮(NO)含量和膜糖蛋白分子的影响。方法实验分为3组(n=12):新鲜液态血小板组(对照1组)、冰冻血小板组(对照2组)、加有GSNO的冷冻血小板组(实验组)。用血小板聚集仪测定冷冻血小板聚集率,用硝酸酶还原法检测NO含量,用流式细胞仪测定冷冻血小板膜糖蛋白分子。结果血小板聚集率(%):对照2组与实验组分别为35.47±2.93 vs 24.43±3.07(P<0.05);NO浓度(μmol/L):对照1组与实验组分别为31.59±16.88 vs 45.64±6.31(P<0.05),对照2组与实验组分别为22.16±6.38 vs 45.64±6.31(P<0.05);膜糖蛋白分子CD42b(%):对照1组与实验组分别为90.46±6.65 vs 78.32±12.54(P<0.05),对照2组与实验组分别为76.94±15.66 vs 78.32±12.54(P>0.05);膜糖蛋白分子CD62P(%):对照1组与实验组分别为12.74±9.64 vs 26.34±9.97(P<0.05),对照2组与实验组分别为31.72±8.20 vs 26.34±9.97(P>0.05)。结论 GSNO增加了冰冻血小板NO的浓度,抑制血小板在贮存过程中的聚集,有望作为冰冻血小板的保护剂。
Objective To investigate the effects of nitrite glutathione (GSNO) on platelet aggregation, nitric oxide (NO) content and membrane glycoprotein. Methods The experiment was divided into three groups (n = 12): fresh liquid platelet group (control group 1), frozen platelet group (control group 2) and frozen platelet group with GSNO (experimental group). Frozen platelet aggregation rate was measured by platelet aggregation analyzer, NO content was detected by nitrate reductase method, frozen platelet membrane glycoprotein was detected by flow cytometry. Results The platelet aggregation rate (%) was 35.47 ± 2.93 vs 24.43 ± 3.07 (P <0.05) in control group 2 and experimental group respectively. The concentration of NO (μmol / L) in control group 1 and experimental group were 31.59 ± 16.88 vs 45.64 ± 6.31, respectively (P <0.05). The control group 2 and experimental group were respectively 22.16 ± 6.38 vs 45.64 ± 6.31 (P <0.05). The percentage of membrane glycoprotein CD42b (%) was 90.46 ± 6.65 78.32 ± 12.54 (P <0.05). The control group 2 and experimental group were 76.94 ± 15.66 vs 78.32 ± 12.54 respectively (P> 0.05). The CD62P (%) of membrane glycoprotein was 12.74 ± 9.64 vs 26.34 ± 9.97 (P <0.05). The control group 2 and experimental group were respectively 31.72 ± 8.20 vs 26.34 ± 9.97 (P> 0.05). Conclusion GSNO increases the concentration of NO in frozen platelets and inhibits the accumulation of platelets during storage, which is expected to serve as a protective agent for frozen platelets.