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目的 探讨丹参对癫痫发作所造成神经元损伤的作用及其机制。方法 采用戊四氮致痫模型 ,运用常规病理及电镜检查方法 ,比较丹参组与对照组神经元受损的情况 ;运用免疫组织化学方法 ,对比观察丹参组与对照组热休克蛋白 (HSP) 70表达的变化。结果 丹参组与对照组动物相比 ,大脑皮质及海马区异常神经元的数目明显减少 (P<0 .0 1) ,超微结构的异常改变也明显减轻。同时丹参组动物脑内 HSP70免疫反应阳性神经元的数目明显增多 (P<0 .0 1) ,染色阳性强度亦增强。结论 丹参能减轻癫痫发作所致的神经元损伤。其作用机制可能与增加HSP70表达有关。
Objective To investigate the effect and mechanism of salvia miltiorrhiza on neuronal damage caused by seizures. Methods Pentylenetetrazole-induced epilepsy model was used to compare the damage of neurons in Salvia miltiorrhiza group and control group by routine pathology and electron microscopy. Immunohistochemical method was used to observe the heat shock protein (HSP) in Salvia miltiorrhiza group and control group. Change in expression. Results Compared with control animals, the number of abnormal neurons in the cerebral cortex and hippocampus was significantly reduced in the Salvia miltiorrhiza group (P<0.01), and abnormal changes in the ultrastructure were also significantly reduced. At the same time, the number of HSP70 immunoreactive neurons in the brain of Salvia miltiorrhiza group was significantly increased (P<0.01), and the positive staining intensity was also increased. Conclusion Salvia miltiorrhiza can reduce neuronal damage caused by seizures. Its mechanism of action may be related to increased HSP70 expression.