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目的:观察体外培养的兔视网膜细胞在受到机械损伤后的形态变化。方法:建立乳兔视网膜细胞及与Schwann Cell(SC)共培养模型,采用机械刮损方法对视网膜细胞造成损伤,相差显微镜下连续24小时观察细胞对损伤的反应。结果:刮损后30分钟胶质细胞逐渐移向损伤区,分裂增殖并填补裸区。神经元突起在损伤后6小时开始再生,其生长方向受到周围环境影响,具有“可塑性”。与SC共培养时,损伤后3小时即可发现再生突起。结论:视网膜神经元突起具有一定再生潜力,胶质增生不是再生的绝对障碍,SC对突起再生具有促进作用。眼科学报2000;16:127-130。
Objective: To observe the morphological changes of cultured rabbit retinal cells after mechanical injury. Methods: Rabbit retinal cells were established and co-cultured with Schwann Cell (SC). The retinal cells were harmed by mechanical scraping. The cells were observed under microscope for 24 hours. Results: Gliocytes gradually migrated to the injured area 30 minutes after the scratch, proliferated and filled the bare area. Neuronal processes begin to regenerate 6 hours after injury, and their growth direction is affected by the surrounding environment, with “plasticity.” When co-cultured with SC, regenerative processes can be found 3 hours after injury. CONCLUSIONS: Retinal neuron projections have some potential for regeneration. Glial proliferation is not an absolute obstacle to regeneration. SC plays a role in promoting neurite regeneration. Journal of Ophthalmology 2000; 16: 127-130.