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我们自1979年1月至1986年10月,对本院遗传咨询门诊的82例原发性闭经患者进行了细胞遗传学检查,现报告如下:材料和方法静脉取血1～2ml,按常规进行外周血淋巴细胞培养及G 带染色体检查,在需证实结构性异染色质的病例,如X Y 核型时,还要作C带核型分析。每例观察30个中期分裂相,镜下分析5个核型,对可疑病例增加分裂相计数和核型分析数,对异常核型至少须有3个G 带核型相同才予以确认。
From January 1979 to October 1986, we conducted a cytogenetic study of 82 patients with primary amenorrhea in the genetic counseling clinic of our hospital. The report is as follows: Materials and Methods Venous blood 1 ~ 2ml, routinely Peripheral blood lymphocyte culture and G-band chromosomal examination, karyotyping of C-band is also required in case of structural heterochromatin to be confirmed, such as XY karyotype. In each case, 30 metaphase were observed, and 5 karyotypes were analyzed microscopically. The numbers of cleavage phase and karyotype analysis were added to suspicious cases, and at least 3 G-band karyotypes should be confirmed for abnormal karyotype.