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目的以免疫缺陷小鼠为受体,探讨受体的性别及完整性对未成熟睾丸组织移植物生长发育的影响。方法将作为受体的免疫缺陷小鼠分为4组:雄性正常组、雄性去势组、雌性正常组和雌性去势组。移植供体组织均为新生1～2d的昆明小鼠睾丸,移植部位为受体背部皮下。移植7周后取材,计算移植物的回收率并称重,对各组移植物中生精小管结构和生精细胞的组成情况以及生精细胞的染色体进行观察分析。结果移植7周后,从4组受体中获得的移植物体积和重量与移植前相比均有明显增加。染色体观察显示,所有4组移植物中生精细胞染色体数量均为正常2倍体(40条)和单倍体(20条);HE染色结果显示,所有4组受体取出的移植物中均发现带有长形精子的生精小管,其中雄性正常组、雄性去势组、雌性正常组和雌性去势组分别有1、5、1和3只受体中观察到含有长形精子的生精小管。结论新生昆明小鼠睾丸组织分别移植到去势的、未去势的雄性和雌性免疫缺陷小鼠背部7周后,未成熟的生精细胞均可发育成为长形精子。
Objective To study the effect of the sex and integrity of the recipient on the growth and development of immature testes tissue in immunodeficient mice. Methods Immunodeficient mice as recipients were divided into 4 groups: male normal group, male castration group, female normal group and female castration group. Transplant donor tissues were freshmen 1 ~ 2d Kunming mice testis, the recipient site subcutaneous subcutaneous. Seven weeks after transplantation, the materials were collected and the graft recovery was calculated and weighed. The structure of seminiferous tubules, the composition of spermatogenic cells and the chromosomes of spermatogenic cells were observed and analyzed. Results After 7 weeks of transplantation, the volume and weight of the grafts obtained from the 4 groups of recipients were significantly increased compared with those before transplantation. Chromosome observation showed that the number of spermatogenic cells in all 4 groups of grafts were normal diploid (40) and haploid (20). The HE staining showed that all the 4 groups of grafts removed the grafts The seminiferous tubules with elongated spermatozoa were found. Among the normal male, male, female and female castration groups, there were 1, 5, 1 and 3 recipients respectively Fine tube. Conclusion The newborn Kunming mice testicular tissue were transplanted to castrated, uncastrated male and female immunodeficient mice 7 weeks after the back, immature spermatogenic cells can develop into long sperm.