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利用cDNA芯片技术从含有2,952个克隆的杨树芯片中筛选出1,160个受杨盘二孢菌诱导的基因。功能分析表明,该1,160个基因分别属于11个功能类别,除了功能未知基因外,参与新陈代谢、防御反应、信号传导及转录调控的基因最多,这4大类基因约占基因总数的42%。1,160个差异表达基因中有926个基因被定位于19条染色体上,其中被定位于第Ⅱ条染色体上的差异基因最多,共102个(11.0%),其次是第Ⅰ条染色体,共93个(10%),被定位到第ⅩⅦ条染色体上的差异基因最少,仅有11个,基因在染色体上的分布则表现为在部分染色体的末端区域存在大量的聚集,在中间区段则相对较少和排列稀疏,基因的这种分布情况与植物抗病的关系有待进一步研究。
1,160 genes induced by Aspergillus terreus were screened from poplar chips containing 2,952 clones using cDNA microarray. The functional analysis showed that the 1,160 genes belonged to 11 functional groups, respectively. Except for unknown function genes, genes involved in metabolism, defense response, signal transduction and transcriptional regulation were the most abundant, accounting for 42% of the total number of genes. Of the 1,160 differentially expressed genes, 926 genes were mapped on 19 chromosomes, of which 102 (11.0%) were located in the second chromosome, followed by the first chromosome, a total of 93 (10%). There were only 11 differential genes mapped to the chromosomes of chromosome ⅩⅦ. The distribution of genes on the chromosomes showed a large number of aggregates in the terminal region of some chromosomes, Less and sparsely arranged, the relationship between the distribution of this gene and plant disease needs further study.