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设计了基于数字微镜器件(DMD)的荧光显微镜探测系统。该系统不仅能对样品实现荧光成像,而且能探测不同区域样品激发荧光信号能量的实时变化。实验采用DMD快速加载多通道不同帧率的视频信息,实现了对激发光束的分割与频率调制。采用光电倍增管采集样品的荧光信息,荧光信息经模/数转换处理后再进行傅里叶变换、滤波和解调操作,得到了不同信道区域荧光信号强度随时间变化的曲线。实验结果表明,将DMD加入到荧光显微镜系统中可以加快集成多通道,且计算机可调控DMD通道,能有效提高荧光显微镜的探测效率。
A fluorescence microscope detection system based on digital micromirror device (DMD) was designed. The system can not only achieve fluorescence imaging of the sample, but also detect the real-time changes of fluorescence signal energy in different regions of the sample. The experiment uses DMD to quickly load multi-channel video information with different frame rates, and realizes the division and frequency modulation of the excitation beam. Fluorescence information was collected by photomultiplier tube. After the fluorescence information was processed by A / D conversion, Fourier transform, filtering and demodulation were used to obtain the curves of fluorescence signal intensity with time in different channel regions. The experimental results show that adding DMD into the fluorescence microscope system can speed up the integration of multi-channel and computer-controlled DMD channels, which can effectively improve the detection efficiency of the fluorescence microscope.