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目的 初步研究 1 甲基 4 苯基 1,2 ,3,6 四氢吡啶 (MPTP)致黑质多巴胺能神经元变性的分子机制。 方法 采用MPTP腹腔注射制备帕金森病褐鼠模型 ,利用mRNA差异显示技术获得在实验组及正常对照组褐鼠黑质中差异表达的基因片段。应用反Northern杂交去除假阳性 ,对真阳性的表达序列标签 (EST)进行克隆、测序及同源性比较。 结果 用 1个随机引物和锚定引物对进行差示反应 ,在实验组获得 2个黑质特异表达的EST ,其中 1个被克隆、测序 ,为 12 1bp ,与人基因abl外显子 1b有低的同源性。 结论 MPTP致黑质神经元变性可能是通过改变促神经元凋亡基因表达而起作用。
Objective To study the molecular mechanism of degeneration of dopaminergic neurons in substantia nigra of 1-methyl-4-phenyl-1,2,6,6-tetrahydropyridine (MPTP). Methods The rat model of Parkinson’s disease was established by intraperitoneal injection of MPTP. The differential display gene fragments in the substantia nigra of experimental group and normal control group were obtained by mRNA differential display. Reverse Northern blotting was used to remove false positives, and the true positive expression sequence tags (ESTs) were cloned, sequenced and their homologies were compared. Results A random primer and a pair of anchored primers were used for differential reaction. Two ESTs with substantia nigra specific expression were obtained in the experimental group, of which 1 was cloned and sequenced to be 12 1bp, which interacted with the human gene abl exon 1b Low homology. Conclusion MPTP-induced degeneration of substantia nigra neurons may play a role by changing the expression of neuronal apoptosis-promoting genes.