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[目的]鉴定弱阳离子磁珠捕获的血清多肽和蛋白峰。[方法]收集经弱阳离子磁珠从人血清中捕获后洗脱下来的蛋白,然后经过HPLC分离,各组分的一部分跑SDS-PAGE胶并用LTQ质谱鉴定,另一部分采用SELDI-TOF检测,最后结合参考文献信息初步确定部分峰的鉴定结果。[结果]通过SELDI-TOF检测结果发现5个组分有比较明显的峰,基于LTQ质谱鉴定了这5个组分分子量在30kD以下的蛋白,数据库搜索结果显示评分在100分以上共鉴定出66个单一蛋白。并初步确定了13个SELDI-TOF上峰的蛋白结果。[结论]HPLC分离富集结合SELDI-TOF和LTQ质谱能比较有效地鉴定丰度比较高的差异峰,是各种差异峰鉴定策略的有益补充。
[Objective] To identify serum peptides and protein peaks captured by weak cationic magnetic beads. [Method] The proteins eluted from human serum by weak cation magnetic beads were collected and then separated by HPLC. A part of each component was run on SDS-PAGE gel and identified by LTQ mass spectrometry. The other part was detected by SELDI-TOF. Finally, According to the reference information, the identification results of partial peaks were preliminarily determined. [Result] The results of SELDI-TOF showed that the five components had obvious peaks. Based on the LTQ mass spectrometry, the proteins whose molecular weights were under 30kD were identified by LTQ mass spectrometry. The database search results showed that the scores were above 100 and 66 A single protein. And initially identified the protein results of 13 peaks on SELDI-TOF. [Conclusion] HPLC separation and enrichment combined with SELDI-TOF and LTQ mass spectrometry can identify differential peaks with high abundance more effectively, which is a useful supplement to the identification of different peaks.