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本文介绍用反相高效色谱分离制备低聚糖单一纯品的方法。色谱分离条件为反相 C18色谱柱,流动相为水体系,检测器采用 RI。在三种不同型号的色谱仪上进行了分离效能的比较。试验证明,国产 YZS-3型制备色谱仪的进样量、分离效能和收率是比较理想的。在样品浓
This article describes the use of reversed-phase high performance liquid chromatography separation of the preparation of a single pure oligosaccharide method. The chromatographic conditions were reversed-phase C18 column, the mobile phase was water and the detector was RI. The separation performance was compared on three different types of chromatographs. Experiments show that the domestic YZS-3 preparation chromatograph injection volume, separation efficiency and yield is ideal. In the sample is thick