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在感染性疾病中，淋巴因子与免疫调节机制密切相关。为了解在人类免疫缺陷病毒（ＨＩＶ）感染患者中淋巴因子对多种刺激原的动态变化反应情况，以便进一步弄清ＨＩＶ感染的病理变化机制，我们采用定量逆转录聚合酶链反应的方法（ＲＴ－ＰＣＲ），对经植物血凝素（ＰＨＡ）、商陆致丝裂素（ＰＷＭ）、念珠菌蛋白（ｃａｎｄｉｄｉｎ）、抗ＣＤ３单克隆抗体（Ａｎｔｉ－ＣＤ３ＭＡｂ）和重组白介素２（ｒＩＬ２）刺激后的ＨＩＶ感染者及对照组外周血单一核细胞（ＰＢＭＣ）中的白介素２（ＩＬ２）、γ干扰素（ＩＦＮ－γ）、白介素１０（ＩＬ１０）、白介素６（ＩＬ６）、肿瘤坏死因子α（ＴＮＦ－α）和ＩＬ２受体（ＩＬ２Ｒ）的动态变化进行了比较。在ＨＩＶ感染组中，其ＩＬ２和ＩＬ２Ｒ基因表达水平明显低于对照组。ＩＬ２和ＩＦＮ－γ均由Ｔ辅助细胞一型（ＴＨ１）克隆产生，然而在ＨＩＶ感染患者中，ＩＦＮ－γ的水平却明显增高。ＩＬ１０基因表达情况视刺激原的不同而不同。经ＰＨＡ、ｃａｎｄｉｄｉｎ、ｒＩＬ２（１０００Ｕ）刺激后，ＨＩＶ感染组ＩＬ１０ｍＲＮＡ表达水平明显高于对照组，但经ｒＩＬ２（１０Ｕ）和Ａｎｔｉ－ＣＤ３ＭＡｂ刺激后却低于对照组。ＨＩＶ感染组ＴＮＦ－α基因表达开?
In infectious diseases, lymphokines and immune regulation are closely related. In order to understand the dynamic response of lymphokines to various stimuli in HIV-infected patients so as to further understand the pathological mechanism of HIV infection, we used quantitative reverse transcriptase-polymerase chain reaction (RT -PCR) was performed on HIV (human immunodeficiency virus) cells after stimulation with phytohaemagglutinin (PHA), merganserinate (PWM), candidin, anti-CD3MAb and rIL2 The levels of IL-2, IFN-γ, IL-10, IL-6 and TNF-α in peripheral blood mononuclear cells (PBMCs) ) And IL2 receptor (IL2R) were compared. In HIV-infected group, IL2 and IL2R gene expression levels were significantly lower than the control group. Both IL2 and IFN-γ are produced by T-helper cell type 1 (TH1) clones, however IFN-γ levels are significantly elevated in HIV-infected patients. IL10 gene expression varies depending on the original stimulus. After stimulation with PHA, candidin and rIL2 (1000 U), the level of IL10mRNA in HIV-infected group was significantly higher than that in control group, but was lower than that in control group after stimulation with rIL2 (10U) and Anti-CD3MAb. TNF-α gene expression in HIV infected group open?