基于硫醇对α,β-不饱和双键的亲核加成,设计合成了一种香豆素-3-氧代-2,3-二氢苯并[b]噻吩加合物的比色硫醇探针.硫醇与探针发生迈克尔加成,打断了分子内的共轭体系,阻止了香豆素向缺电子的3-氧代-2,3-二氢苯并[b]噻吩基团的分子内电荷转移,引起了吸收光谱上的蓝移和溶液颜色的变化. Based on the nucleophilic addition of thiol to α, β-unsaturated double bonds, a colorimetric coumarin-3-oxo-2,3-dihydrobenzo [b] thiophene adduct A thiol probe. Michael addition of the thiol to the probe interrupts the intramolecular conjugation system and inhibits coumarin from electron-deficient 3-oxo-2,3-dihydrobenzo [b] The intramolecular charge transfer of the thienyl group caused a blue shift in the absorption spectrum and a change in the color of the solution.