南京军区总医院继在国內用自制的培养基分离出第一株军团病杆菌后,在杨毓华副主任技师和黄宇峰技师的努力下,又研制成功了军团病杆菌“种”特异性单克隆抗体和抗人α_2-巨球蛋白单克隆抗体,并于1985年4月14日通过鉴定。此种单克隆抗体用来检测1～6型军团病杆菌具有快速、简便、特异性高等优点,与美国疾病控制中心提供的1～6型标准军团病杆菌均出现强阳性交叉反应,而与金黄色葡萄球菌、肺炎杆菌、大肠杆菌、痢疾杆菌、绿脓杆菌以及产气杆 Nanjing Military Region General Hospital following the first homemade strain of Legionella in China using homemade medium, with the efforts of Yang Yuhua, deputy chief technician and Huang Yufeng technicians, the successful development of Legionella species “specific” monoclonal antibody And anti-human α_2-macroglobulin monoclonal antibody, and in April 14, 1985 through the identification. The monoclonal antibody used to detect Legionella pneumophila types 1 to 6 has the advantages of fast, simple and high specificity, and strongly positive cross-reactivity with the type 1 to 6 standard Legionella isolates provided by the U.S. Centers for Disease Control and Prevention, Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli, Shigella dysenteriae, Pseudomonas aeruginosa and gas producing rod