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目的:研究IL-24在子痫前期胎盘的表达及rhIL-24对TEV-1细胞MMP-2蛋白活性表达的影响,探讨二者与子痫前期发病机制的关系。方法:RT-PCR检测子痫前期及正常足月孕妇胎盘中IL-24 mRNA的表达;明胶酶谱检测0、10、50、100、500ng/mlrhIL-24作用后TEV-1细胞的MMP-2活性;Western blot检测不同浓度rhIL-24作用后TEV-1细胞的MMP-2蛋白表达。结果:子痫前期组胎盘组织中IL-24 mRNA表达显著高于正常足月组(P<0.05)。当rhIL-24浓度≥50ng/ml后,TEV-1细胞中MMP-2蛋白活性表达均明显降低(P<0.05),并呈一定的浓度依赖性。结论:IL-24异常升高,抑制了滋养细胞MMP-2的分泌及活性,使其侵袭力下降,从而在子痫前期发病中起一定作用。
Objective: To investigate the effect of IL-24 on placenta preeclampsia and the effect of rhIL-24 on the expression of MMP-2 protein in TEV-1 cells and the relationship between them and the pathogenesis of preeclampsia. Methods: The expression of IL-24 mRNA in placenta of preeclampsia and normal term pregnant women was detected by RT-PCR. The gelatin zymography was used to detect the expression of MMP-2 in TEV-1 cells after treated with 0, 10, 50, 100 and 500ng / ml rhIL- The protein expression of MMP-2 in TEV-1 cells treated with different concentrations of rhIL-24 was detected by Western blot. Results: The expression of IL-24 mRNA in placenta of preeclampsia group was significantly higher than that in normal term group (P <0.05). When the concentration of rhIL-24≥50ng / ml, the expression of MMP-2 protein in TEV-1 cells was significantly decreased (P <0.05), and in a concentration-dependent manner. CONCLUSION: Abnormal increase of IL-24 inhibits the secretion and activity of MMP-2 in trophoblast and decreases the invasiveness of IL-24, which may play a role in the pathogenesis of preeclampsia.