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为探讨ＥＢ病毒（ＥＢＶ）感染与胃癌（ＧＣ）发生的关系，作者应用原位聚合酶链反应（ＰＣＲＩＳ）及一步反转录（ＯＲＴ－ＰＣＲＩＳ）对Ｇｃ、慢性萎缩性胃炎（ＣＡＧ）、慢性浅表性胃炎（ＣＳＧ）３组病例１３６份组织标本进行ＥＢＶＤＮＡ或ＥＢＶＲＮＡ检测。发现ＥＢＶ在ＧＣ组检出率达２９．６％（６１５４）、ＣＡＧ组为１１．６％（５／４３），两组差异显著（Ｐ＜０．０５）。而ＣＳＧ组全部阴性；在ＧＣ组中ＨＢＶＤＮＡ阳性率为２７．７％，明显高于ＥＢＶＤＮＡ的１４．８％（Ｐ＜０．０５）。结果提示，ＥＢＶ感染与ＧＣ发生存在某种相关。ＥＢＶ基因于一些癌细胞及癌前病变组织内存在或以ＲＮＡ形式表达的实际意义有待进一步探讨。
To investigate the relationship between EBV infection and gastric cancer (GC), the authors used in situ polymerase chain reaction (PCRIS) and one-step reverse transcription (ORT-PCRIS) for Gc, chronic atrophic gastritis (CAG), and chronic 136 cases of tissue samples from 3 groups of superficial gastritis (CSG) were tested for EBV DNA or EBV RNA. The detection rate of EBV in the GC group was 29.6% (6154) and CAG group was 11.6% (5/43). There was a significant difference between the two groups (P<0.05). The CSG group was all negative; in the GC group, the positive rate of HBVDNA was 27.7%, which was significantly higher than that of EBV DNA (14.8%) (P<0.05). The results suggest that there is some correlation between EBV infection and GC. The actual significance of EBV gene expression in some cancer cells and precancerous lesions or in the form of RNA remains to be further explored.