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比较了马尾松毛虫核型多角体病毒多角体蛋白（ＤｐＮＰＶ－ＰＰ）这种昆虫病毒蛋白在ｐＨ值不同的银胶溶液中的表面增强拉曼光谱。在中ｐＨ值溶液中，ＤｐＮＰＶ－ＰＰ主要通过硫氢基团和羧基基团吸附于银表面。在低ｐＨ值溶液中，硫氢基团的吸附作用趋弱，ＤｐＮＰＶ－ＰＰ主要通过羧基基团和氨基基团与银粒子表面键联，并以阴离子构型［ＮＨ２ＲＣＯＯ－］存在于溶液中。而在高ｐＨ值溶液中，仅有羧基基团与银表面的吸附作用较强。不同ｐＨ值条件下，低波数带２３８ｃｍ－１～２２６ｃｍ－１的出现，均表明ＤｐＮＰＶ－ＰＰ与银表面产生明显的化学吸附。ＤｐＮＰＶ－ＰＰ在银胶中的增强属于分子增强机制，具有短程作用特性。银胶溶液ｐＨ值的改变，引起被吸附基团与银表面之间的吸附方式、相对距离、相对几何状态等因素发生改变，导致了不同ｐＨ值条件下拉曼散射增强效果的不同。
The surface-enhanced Raman spectra of the insect virus proteins of Dendrolimus punctatus nucleopolyhedrovirus polyhedrin (DpNPV-PP) at different pH values were compared. In the medium pH solution, DpNPV-PP mainly adsorbed to the silver surface through thiol groups and carboxyl groups. In the low pH solution, the adsorption of sulfhydryl groups weakens. DpNPV-PP is mainly bonded to the surface of silver particles via carboxyl group and amino group, and exists in solution in anionic configuration [NH2RCOO-]. However, in high pH solution, only the carboxyl groups adsorbed strongly on silver surface. Under the different pH conditions, the presence of 238cm-1 ~ 226cm-1 in the low wave number band showed that DpNPV-PP produced obvious chemisorption on silver surface. The enhancement of DpNPV-PP in silver colloid is a molecular enhancement mechanism with short-range properties. The change of the pH value of the silver colloid solution causes the adsorption mode, relative distance, relative geometric state and other factors to change between the adsorbed groups and the silver surface, resulting in different Raman scattering enhancement effects under different pH values.